Development of micro-flow devices by direct-milling on poly(methyl methacrylate) substrates with integrated optical detection

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

Eunice Rodrigues (Autor) FFUP; Rui Lapa (Autor) FFUP

Revista

Título: Mikrochimica Acta

Vol. 166 Nº 3

Páginas: 189-195

ISSN: 0026-3672

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 7 Citações

ISI Web of Science

Scopus - 8 Citações

Pubmed / Medline

Classificação Científica

FOS: Ciências médicas e da saúde > Outras ciências médicas

CORDIS: Ciências da Saúde

Outras Informações

ID Authenticus: P-003-GBM

Resumo (PT): The development of micro-flow devices by direct-milling on poly(methyl methacrylate) (PMMA) substrates and incorporating the optic detector into the same structure is presented, evaluated and applied to the determination of chlorhexidine. Hydrodynamic characteristics of the developed devices were compared against those obtained from flow systems based on classic Teflon reactors. The optical detector integrated in the PMMA substrate is composed of a light emitting diode (λ = 627 nm) and light dependence resistance as light source and light detector, respectively. The performance of the optical detector was evaluated and optimized. The applicability of the fluidic device was assessed in the determination of chlorhexidine through its colorimetric reaction with bromocresol green. Micro-pumps were used as propulsion units. Results obtained in real sample analysis were in good agreement with those obtained by the reference procedure. >br> <br> Keywords Microfluidic device - PMMA - Direct-milling - Optic detector integration - Multi-pumping - Chlorhexidine <br> <a target="_blank" href="http://www.springerlink.com/content/lt08g29374631271/?p=74bd3aeed6ba4beab2896ea6b875fe6e&pi=0"> Texto integral </a> <br> <br>

Abstract (EN): The development of micro-flow devices by direct-milling on poly(methyl methacrylate) (PMMA) substrates and incorporating the optic detector into the same structure is presented, evaluated and applied to the determination of chlorhexidine. Hydrodynamic characteristics of the developed devices were compared against those obtained from flow systems based on classic Teflon reactors. The optical detector integrated in the PMMA substrate is composed of a light emitting diode (λ = 627 nm) and light dependence resistance as light source and light detector, respectively. The performance of the optical detector was evaluated and optimized. The applicability of the fluidic device was assessed in the determination of chlorhexidine through its colorimetric reaction with bromocresol green. Micro-pumps were used as propulsion units. Results obtained in real sample analysis were in good agreement with those obtained by the reference procedure. <br> <br> Keywords Microfluidic device - PMMA - Direct-milling - Optic detector integration - Multi-pumping - Chlorhexidine <br> <a target="_blank" href="http://www.springerlink.com/content/lt08g29374631271/?p=74bd3aeed6ba4beab2896ea6b875fe6e&pi=0"> Full text </a> <br> <br>

Idioma: Português

Tipo (Avaliação Docente): Científica