Development of micro-flow devices by direct-milling on poly(methyl methacrylate) substrates with integrated optical detection

• Type: Article in International Scientific Journal
• Date: 2009

Authors

Eunice Rodrigues (Author) FFUP; Rui Lapa (Author) FFUP

Journal

Title: Mikrochimica Acta

Vol. 166 No. 3

Pages: 189-195

ISSN: 0026-3672

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 7 Citations

ISI Web of Science

Scopus - 8 Citations

Pubmed / Medline

Scientific classification

FOS: Medical and Health sciences > Other medical sciences

CORDIS: Health sciences

Other information

Authenticus ID: P-003-GBM

Resumo (PT): The development of micro-flow devices by direct-milling on poly(methyl methacrylate) (PMMA) substrates and incorporating the optic detector into the same structure is presented, evaluated and applied to the determination of chlorhexidine. Hydrodynamic characteristics of the developed devices were compared against those obtained from flow systems based on classic Teflon reactors. The optical detector integrated in the PMMA substrate is composed of a light emitting diode (λ = 627 nm) and light dependence resistance as light source and light detector, respectively. The performance of the optical detector was evaluated and optimized. The applicability of the fluidic device was assessed in the determination of chlorhexidine through its colorimetric reaction with bromocresol green. Micro-pumps were used as propulsion units. Results obtained in real sample analysis were in good agreement with those obtained by the reference procedure. >br> <br> Keywords Microfluidic device - PMMA - Direct-milling - Optic detector integration - Multi-pumping - Chlorhexidine <br> <a target="_blank" href="http://www.springerlink.com/content/lt08g29374631271/?p=74bd3aeed6ba4beab2896ea6b875fe6e&pi=0"> Texto integral </a> <br> <br>

Abstract (EN): The development of micro-flow devices by direct-milling on poly(methyl methacrylate) (PMMA) substrates and incorporating the optic detector into the same structure is presented, evaluated and applied to the determination of chlorhexidine. Hydrodynamic characteristics of the developed devices were compared against those obtained from flow systems based on classic Teflon reactors. The optical detector integrated in the PMMA substrate is composed of a light emitting diode (λ = 627 nm) and light dependence resistance as light source and light detector, respectively. The performance of the optical detector was evaluated and optimized. The applicability of the fluidic device was assessed in the determination of chlorhexidine through its colorimetric reaction with bromocresol green. Micro-pumps were used as propulsion units. Results obtained in real sample analysis were in good agreement with those obtained by the reference procedure. <br> <br> Keywords Microfluidic device - PMMA - Direct-milling - Optic detector integration - Multi-pumping - Chlorhexidine <br> <a target="_blank" href="http://www.springerlink.com/content/lt08g29374631271/?p=74bd3aeed6ba4beab2896ea6b875fe6e&pi=0"> Full text </a> <br> <br>

Language: Portuguese

Type (Professor's evaluation): Scientific