DODECYLGUANIDINE MONOACETATE (DODINE) CAUSES SEVERE MEMBRANE DAMAGE IN PSEUDOMONAS-SYRINGAE ABOVE THE CRITICAL MICELLE CONCENTRATION

• Tipo: Artigo em Revista Científica Internacional
• Data: 1993

Autores

cabral, jps (Autor) FCUP

Revista

Título: Journal of Basic Microbiology

Vol. 33

Páginas: 219-225

ISSN: 0233-111X

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 6 Citações

Scopus - 7 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-001-NNA

DOI: 10.1002/jobm.3620330402

Abstract (EN): The release of K+ from Pseudomonas syringae cells treated with dodecylguanidine monoacetate (dodine) was followed with a K+-selective glass electrode. Treatment of the cells with 5 - 15 mumol/l dodine resulted in low levels of K+ release, but higher surfactant concentrations caused extensive and rapid K+ efflux. Dodine concentrations that caused high K+ release also induced significant leakage of inorganic phosphate. The addition of 5 - 10 mumol/l dodine also caused an increase in the rate of oxygen consumption in the presence of glycerol or succinate, but an increase in concentration from 10 to 40 mumol/l resulted in a concomitant decrease in O2 consumption. The results from this and previous work suggest that dodine inhibits respiration firstly by causing drainage of coenzymes, and then by a direct interaction with the components of the respiratory chain. Previous work showed that above 25 mumol/l, dodine molecules aggregate to form micelles. The results therefore suggests that, in contrast with other cationic amphiphiles, the micellar form of dodine is more damaging to the cytoplasmic membrane than the free molecules.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7