pH opposite effects on synthesis of dinucleoside polyphosphates and on oxidation reactions catalyzed by firefly luciferase

• Tipo: Artigo em Revista Científica Internacional
• Data: 2003

Autores

fraga, h (Autor) Outra; da silva, jcge (Autor) FCUP; fontes, r (Autor) FMUP

Revista

Título: FEBS Letters

Vol. 543

Páginas: 37-41

ISSN: 0014-5793

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 17 Citações

Scopus - 17 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-000-GM0

DOI: 10.1016/s0014-5793(03)00382-x

Abstract (EN): Previous results have shown that an oxidizing product of firefly luciferin, dehydroluciferyl-adenylate, is the main intermediate in the process of synthesis of dinucleoside polyphosphates catalyzed by firefly luciferase (EC 1.13.12.7). However, we have found that the pH effects on the luciferase oxidizing processes and on the synthesis of dinucleoside polyphosphate are opposite: acidic assay media enhance the synthesis of dinucleoside polyphosphate and inhibit the oxidizing processes. The reason for this apparent contradiction lies on the activation effect of low pH on the adenylate transfer reaction from dehydroluciferyl-adenylate to the acceptor nucleotide. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5