Quantitative analysis of kielcorins in biomimetic synthesis by liquid chromatography/UV detection

• Tipo: Artigo em Revista Científica Internacional
• Data: 2003

Autores

Sousa, ME (Autor) Outra; Afonso, CMM (Autor) FFUP; Pinto, MMM (Autor) FFUP

Revista

Título: Journal of Liquid Chromatography and Related Technologies

Vol. 26

Páginas: 29-41

ISSN: 1082-6076

Editora: Taylor & Francis

Indexação

ISI Proceedings

ISI Web of Knowledge - 3 Citações

Scopus - 4 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-000-K16

DOI: 10.1081/jlc-120017150

Abstract (EN): An HPLC/UV assay was developed for separation and quantification of isomeric kielcorins in different synthetic crude products. trans(+/-)-Kielcorin C, cis-(+/-)-kielcorin C, trans-(+/-)-kielcorin D, trans-(+/-)isokielcorin D, and trans-(+/-)-kielcorin E were obtained by oxidative coupling of coniferyl alcohol and an appropriate xanthonic building block, 3,4-, 1,2-, and 2,3-dihydroxyxanthone, respectively, with silver carbonate, silver oxide, and potassium hexacyanoferrate (III). The isomeric kielcorins were separated and quantified directly in the nine crude products obtained. The assay was validated with respect to specificity, linearity, range, limits of quantification, and detection.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 13