In Vitro Protective Effect of Hypericum androsaemum Extract Against Oxygen and Nitrogen Reactive Species

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

Isabel F Almeida (Autor) FFUP; Eduarda Fernandes (Autor) FFUP; Jose L F C Lima (Autor) FFUP; Paulo Cardoso Costa (Autor) FFUP; Maria Fernanda Bahia (Autor) Outra

Revista

Título: Basic and Clinical Pharmacology and Toxicology

Vol. 105

Páginas: 222-227

ISSN: 1742-7835

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 13 Citações

Scopus - 13 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina básica

Outras Informações

ID Authenticus: P-003-F5F

DOI: 10.1111/j.1742-7843.2009.00458.x

Abstract (EN): Hypericum androsaemum L. (Gutiferae) is a medicinal plant growing in Western Europe that has been used in traditional medicine in the prevention or treatment of liver diseases. Oxidative stress and nitrosative stress are common pathogenetic mechanisms contributing to initiation and progression of hepatic damage in several liver disorders. In the present study, an ethanol:water (4:6) extract from H. androsaemum branches and leaves were evaluated for its putative in vitro scavenging effects on 1,1-diphenyl-2-picrylhydrazil radical, on reactive oxygen species, namely HO<SU center dot</SU, O(2)<SU center dot-</SU, ROO<SU center dot</SU, <SU1</SUO(2) and H(2)O(2) and on reactive nitrogen species, namely <SU center dot</SUNO and ONOO<SU-</SU. The hypericum extract presented a remarkable capacity to scavenge all the tested reactive species, all the IC(50) values being found at the mu g/ml level. IC(50) values for 1,1-diphenyl-2-picrylhydrazil, and for the reactive oxygen species O(2)<SU center dot-</SU, H(2)O(2,) HO<SU center dot</SU and <SU1</SUO(2) were 11.3 +/- 0.7, 32.7 +/- 3.4, 944 +/- 47, 595 +/- 82, 28.3 +/- 1.2 mu g/ml respectively. The oxygen radical absorbance capacity value obtained for ROO<SU center dot</SU was 1.5 +/- 0.1 mu mol Trolox equivalents/mg extract. The IC(50) values for <SU center dot</SUNO and ONOO<SU-</SU were 2.2 +/- 0.2 and 1.2 +/- 0.1 mu g/ml respectively. The content of total phenolics was 281 +/- 2 mg of gallic acid equivalents/g of lyophilized extract. The observed antioxidant activity provides scientific support for the reported therapeutic use of H. androsaemum, though further in vitro and in vivo studies are required to ascertain the risk/benefit score at therapeutic concentrations.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6