Development of an HPLC/diode-array/fluorimetric detector method for monitoring tocopherols and tocotrienols in edible oils

• Tipo: Artigo em Revista Científica Internacional
• Data: 2000

Autores

Gama, P (Autor) Outra; Casal, S (Autor) FFUP; Oliveira, B (Autor) FFUP; Ferreira, MA (Autor) Outra

Revista

Título: Journal of Liquid Chromatography and Related Technologies

Vol. 23

Páginas: 3011-3022

ISSN: 1082-6076

Editora: Taylor & Francis

Indexação

ISI Web of Knowledge - 27 Citações

Scopus - 30 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-001-1SB

DOI: 10.1081/jlc-100101839

Abstract (EN): This paper describes an HPLC procedure for the determination of tocopherols and tocotrienols in crude and edible vegetable oils. The sample preparation only involves a dissolution of the oil in n-hexane and filtration. The chromatographic separation was achieved using a normal phase column LiChrosorb SI 60 (5 mum; 25.0 x 0.4 cm). Isocratic elution was carried out using n-hexane :2-propanol (99.7:0.3). The effluent was monitored by using diode-array and fluorimetric detectors. The determinations were performed in the following linear ranges: 0.5-7.5 mug/mL for alpha -tocopherol and beta -tocotrienol; 0.5-10 mug/mL for beta -tocopherol; and 0.5-15 mug/mL for alpha -tocotrienol, gamma -tocopherol, gamma -tocotrienol, and delta -tocopherol. Extensive quality assurance of the proposed method was performed by the standard addition method in both crude and edible oil. For edible oil, the precision obtained (n=10) was better than 1.8, 2.0, and 1.4 CV% for alpha, beta, and gamma -tocopherol, respectively; for crude oil it was better than 1.9, 2.4, and 2.4% for alpha, beta, and gamma -tocopherol, respectively. Furthermore, for edible oil the mean recovery values were between 96-116%, 58-69%, and 111-115% for alpha, beta, and gamma -tocopherol, respectively; for crude oil recovery values were between 85-95%, 63-66%, and 99-103% for the same tocopherols, respectively. The proposed method appears to be an adequate method for quality control and helpful for authenticity verification by the official control in oil industry.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12