Application of amperometric sol-gel biosensor to flow injection determination of glucose

• Tipo: Artigo em Revista Científica Internacional
• Data: 2002

Autores

Couto, CMCM (Autor) Outra; Araujo, AN (Autor) FFUP; Montenegro, MCBSM (Autor) FFUP; Rohwedder, J (Autor) Outra; Raimundo, I (Autor) Outra; Pasquini, C (Autor) Outra

Revista

Título: Talanta

Vol. 56 Nº 6

Páginas: 997-1003

ISSN: 0039-9140

Editora: Elsevier

Indexação

ISI Web of Knowledge - 28 Citações

ISI Web of Science

Scopus - 31 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

CORDIS: Ciências da Saúde

Outras Informações

ID Authenticus: P-000-PQ9

DOI: 10.1016/s0039-9140(01)00587-2

Resumo (PT): A glucose biosensor with enzyme immobilised by sol–gel technology was constructed and evaluated. The glucose biosensor reported is based on encapsulated GOX within a sol–gel glass, prepared with 3-aminopropyltriethoxy silane, 2-(3,4-epoxycyclohexyl)-ethyltrimetoxy silane and HCl. A flow system incorporating the amperometric biosensor constructed was developed for the determination of glucose in the 1×10−4–5×10−3 mol l−1 range with a precision of 1.5%. The results obtained for the analysis of electrolytic solution for iv administration and human serum samples showed good agreement between the proposed method and the reference procedure, with relative error <5%. <br> <br> Keywords: Flow injection analysis; Glucose oxidase; Sol–gel; Glucose biosensor <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6THP-44CY0W6-1&_user=2460038&_coverDate=04%2F08%2F2002&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=56a4448db9dcee9b2e0f7eecabac5b15"> Texto integral </a> <br> <br>

Abstract (EN): A glucose biosensor with enzyme immobilised by sol-gel technology was constructed and evaluated. The glucose biosensor reported is based on encapsulated GOX within a sol-gel glass, prepared with 3-aminopropyltriethoxy silane, 2-(3,4-epoxycyclohexyl)-ethyltrimetoxy silane and HCl. A flow system incorporating the amperometric biosensor constructed was developed for the determination of glucose in the 1 x 10(-4)-5 x 10(-3) mol 1(-1) range with a precision of 1.5%. The results obtained for the analysis of electrolytic solution for iv administration and human serum samples showed good agreement between the proposed method and the reference procedure, with relative error < 5%.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7