Wild mushrooms Clitocybe alexandri and Lepista inversa: In vitro antioxidant activity and growth inhibition of human tumour cell lines

• Tipo: Artigo em Revista Científica Internacional
• Data: 2010

Autores

Josiana A Vaz (Autor) Outra; Sandrina A Helen (Autor) Outra; Anabela Martins (Autor) Outra; Gabriela M Almeida (Autor) Outra; Helena H Vasconcelos (Autor) FFUP; Isabel C F R Ferreira (Autor) Outra

Revista

Título: Food and Chemical Toxicology

Vol. 48 Nº 10

Páginas: 2881-2884

ISSN: 0278-6915

Editora: Elsevier

Indexação

ISI Web of Knowledge - 72 Citações

ISI Web of Science

Scopus - 81 Citações

Pubmed / Medline

Classificação Científica

FOS: Ciências agrárias > Outras ciências agrárias

CORDIS: Ciências da Saúde > Ciências farmacológicas > Farmácia

Outras Informações

ID Authenticus: P-003-2CR

DOI: 10.1016/j.fct.2010.07.021

Resumo (PT): The in vitro antioxidant and growth inhibitory activity of extracts obtained from two Portuguese wild mushrooms (Clitocybe alexandri and Lepista inversa) was studied in human tumour cell lines. The extracts were phenolic (methanolic and ethanolic) and polysaccharidic (boiling water). The antioxidant activity assays included evaluation of radical-scavenging capacity, reducing power and inhibition of lipid peroxidation measured in liposome solutions. Extract-induced cell growth inhibition was measured in four different tumour cell lines (lung, breast, colon and gastric cancer) using the SRB assay. The polysaccharidic extract of L. inversa was the most potent as antioxidant (EC50 < 1.8 ± 0.1 mg/ml), while the phenolic ethanolic extract of C. alexandri was the most potent as inhibitor of growth of the studied cancer cell lines (GI50 < 26.0 ± 1.3 μg/ml). Together, these activities indicate that these mushrooms are promising sources of bioactive compounds. <br> <br> Keywords: Wild mushrooms; Antioxidant activity; Growth inhibitory activity <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T6P-50JPNCR-2&_user=2460038&_coverDate=10%2F31%2F2010&_rdoc=49&_fmt=high&_orig=browse&_origin=browse&_zone=rslt_list_item&_srch=doc-info(%23toc%235036%232010%23999519989%232353770%23FLA%23display%23Volume)&_cdi=5036&_sort=d&_docanchor=&_ct=71&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=7abd62e9cb6fa83a5707dfe82116a2d0&searchtype=a "> Texto integral </a> <br> <br>

Abstract (EN): The in vitro antioxidant and growth inhibitory activity of extracts obtained from two Portuguese wild mushrooms (Clitocybe alexandri and Lepista inversa) was studied in human tumour cell lines. The extracts were phenolic (methanolic and ethanolic) and polysaccharidic (boiling water). The antioxidant activity assays included evaluation of radical-scavenging capacity, reducing power and inhibition of lipid peroxidation measured in liposome solutions. Extract-induced cell growth inhibition was measured in four different tumour cell lines (lung, breast, colon and gastric cancer) using the SRB assay. The polysaccharidic extract oft. inversa was the most potent as antioxidant (EC(50) < 1.8 +/- 0.1 mg/ml), while the phenolic ethanolic extract of C alexandri was the most potent as inhibitor of growth of the studied cancer cell lines (Gl(50) <26.0 +/- 1.3 mu g/ml). Together, these activities indicate that these mushrooms are promising sources of bioactive compounds.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 4