Resumo (PT):
A micromethod was developed and validated for the quantification of lutein and β-carotene in olive oils. The compounds are liquid-liquid extracted with n-hexane and n,n-dimethylformamide (DMF), by vortex mixing and centrifugation. The n-hexane phase retains β-carotene, whereas the DMF phase retains lutein. This last fraction is concentrated and analyzed by RP-HPLC/diode-array, with a gradient of acetonitrile, water, and ethyl acetate, using β-apo-8'-carotenal as I.S. β-carotene is quantified by UV/Vis measurements at 454 nm, by the external standard method. The method shows appropriate inter- and intra-day reproducibility, accuracy, and linearity for both compounds, allowing fast analysis of several samples simultaneously, with a relatively small consumption of organic solvents. The analytical methodology was applied to several samples of commercial Portuguese PDO olive oils.
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Keywords: Olive oil; RP-HPLC; Lutein; β-Carotene; Internal standard; Micromethod
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<a target="_blank" href="http://web.ebscohost.com/ehost/detail?vid=4&hid=103&sid=aed51d49-d746-43fd-8e5b-150fd7bc5820%40sessionmgr109"> Texto integral </a>
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Abstract (EN):
A micromethod was developed and validated for the quantification of lutein and β-carotene in olive oils. The compounds are liquid-liquid extracted with n-hexane and n,n-dimethylformamide (DMF), by vortex mixing and centrifugation. The n-hexane phase retains β-carotene, whereas the DMF phase retains lutein. This last fraction is concentrated and analyzed by RP-HPLC/diode-array, with a gradient of acetonitrile, water, and ethyl acetate, using β-apo-8'-carotenal as I.S. β-carotene is quantified by UV/Vis measurements at 454 nm, by the external standard method. The method shows appropriate inter- and intra-day reproducibility, accuracy, and linearity for both compounds, allowing fast analysis of several samples simultaneously, with a relatively small consumption of organic solvents. The analytical methodology was applied to several samples of commercial Portuguese PDO olive oils.
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Keywords: Olive oil; RP-HPLC; Lutein; β-Carotene; Internal standard; Micromethod
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<a target="_blank" href="http://web.ebscohost.com/ehost/detail?vid=4&hid=103&sid=aed51d49-d746-43fd-8e5b-150fd7bc5820%40sessionmgr109"> Full text </a>
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Idioma:
Português
Tipo (Avaliação Docente):
Científica