Transthyretin interacts with actin regulators in a Drosophila model of familial amyloid polyneuropathy

• Tipo: Artigo em Revista Científica Internacional
• Data: 2020

Autores

Oliveira da Silva, MIO (Autor) Outra; Lopes, CS (Autor) Outra; Liz, MA (Autor) Outra

Revista

Título: Scientific Reports

Vol. 10

ISSN: 2045-2322

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 1 Citação

Outras Informações

ID Authenticus: P-00S-KTM

DOI: 10.1038/s41598-020-70377-4

Abstract (EN): Familial amyloid polyneuropathy (FAP) is a neurodegenerative disorder whose major hallmark is the deposition of mutated transthyretin (TTR) in the form of amyloid fibrils in the peripheral nervous system (PNS). The exposure of PNS axons to extracellular TTR deposits leads to an axonopathy that culminates in neuronal death. However, the molecular mechanisms underlying TTR-induced neurodegeneration are still unclear, despite the extensive studies in vertebrate models. In this work we used a Drosophila FAP model, based on the expression of the amyloidogenic TTR (V30M) in the fly retina, to uncover genetic interactions with cytoskeleton regulators. We show that TTR interacts with actin regulators and induces cytoskeleton alterations, leading to axonal defects. Moreover, our study pinpoints an interaction between TTRV30M and members of Rho GTPase signaling pathways, the major actin regulators. Based on these findings we propose that actin cytoskeleton alterations may mediate the axonopathy observed in FAP patients, and highlight a molecular pathway, mediated by Rho GTPases, underlying TTR-induced neurodegeneration. We expect this work to prompt novel studies and approaches towards FAP therapy.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9