Characterization of planktonic and biofilm cells from two filamentous cyanobacteria using a shotgun proteomic approach

• Tipo: Artigo em Revista Científica Internacional
• Data: 2020

Autores

Maria João Romeu (Autor) FEUP; Dany Domínguez-Pérez (Autor) Outra; Daniela Almeida (Autor) Outra; João Morais (Autor) Outra; Alexandre Campos (Autor) Outra; Vítor Vasconcelos (Autor) Outra; Filipe Mergulhão (Autor) FEUP

Revista

Título: Biofouling

Vol. 36 Nº 6

Páginas: 631-645

ISSN: 0892-7014

Editora: Taylor & Francis

Indexação

ISI Web of Knowledge - 10 Citações

Scopus - 13 Citações

Projetos Associados

CVMar+i - Inovação industrial através de colaborações específicas entre empresas e centros de investigação no contexto de valorização biotecnológica marinha

LEPABE - Projeto Estratégico do LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Outras Informações

ID Authenticus: P-00S-GJW

DOI: 10.1080/08927014.2020.1795141

Abstract (EN): Cyanobacteria promote marine biofouling with significant impacts. A qualitative proteomic analysis, by LC-MS/MS, of planktonic and biofilm cells from two cyanobacteria was performed. Biofilms were formed on glass and perspex at two relevant hydrodynamic conditions for marine environments (average shear rates of 4 s(-1)and 40 s(-1)). For both strains and surfaces, biofilm development was higher at 4 s(-1). Biofilm development ofNodosilineasp. LEGE 06145 was substantially higher thanNodosilineasp. LEGE 06119, but no significant differences were found between surfaces. Overall, 377 and 301 different proteins were identified forNodosilineasp. LEGE 06145 andNodosilineasp. LEGE 06119. Differences in protein composition were more noticeable in biofilms formed under different hydrodynamic conditions than in those formed on different surfaces. Ribosomal and photosynthetic proteins were identified in most conditions. The characterization performed gives new insights into how shear rate and surface affect the planktonic to biofilm transition, from a structural and proteomics perspective.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 15