Rheological behaviour of vitreous humour

• Tipo: Artigo em Revista Científica Internacional
• Data: 2017

Autores

Silva, AF (Autor) Outra; Alves, MA (Autor) FEUP; Mónica S. N. Oliveira (Autor) FEUP

Revista

Título: Rheologica Acta

Vol. 56

Páginas: 377-386

ISSN: 0035-4511

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 11 Citações

Scopus - 67 Citações

Outras Informações

ID Authenticus: P-00M-PX3

DOI: 10.1007/s00397-017-0997-0

Abstract (EN): The vitreous humour (VH) is a complex biofluid that occupies a large portion of the eyeball between the lens and the retina, and exhibits non-Newtonian rheological properties that are key for its function in the eye. It is often possible to distinguish two different phases in VH, known as liquid and gel phases (Sebag J Eye 1: 254-262, 1987). In this work, we present a detailed rheological characterisation of the two phases of the VH under shear and extensional flow conditions. Healthy New Zealand rabbit eyes were used to measure the surface tension and the shear and extensional rheological properties of VH in different phase conformations and at different times after dissection. The results show that VH liquid phase exhibits a surface tension of 47.8 mN/m, a shear thinning behaviour reaching a viscosity plateau around 10(-3) Pa s for shear rates above similar to 1000 s(-1), and an average relaxation time of 9.7 ms in extensional flow. Interestingly, both VH phases present higher storage modulus than loss modulus, and the measurements performed with VH gel phase 4 +/- 1 h after dissection exhibit the highest moduli values. The compliance measurements for the gel phase show a viscoelastic gel behaviour and that compliance values decrease substantially with time after dissection. Our results show that the two VH phases exhibit viscoelastic behaviour, but with distinct rheological characteristics, consistent with a gel phase mostly composed of collagen entangled by hyaluronan and a second phase mainly composed of hyaluronan in aqueous solution.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10