Apo E-Functionalization of Solid Lipid Nanoparticles Enhances Brain Drug Delivery: Uptake Mechanism and Transport Pathways

• Tipo: Artigo em Revista Científica Internacional
• Data: 2017

Autores

Neves, AR (Autor) Outra; Queiroz, JF (Autor) Outra; Lima, SAC (Autor) Outra; Salette Reis (Autor) FFUP

Revista

Título: Bioconjugate Chemistry

Vol. 28

Páginas: 995-1004

ISSN: 1043-1802

Editora: American Chemical Society

Indexação

ISI Web of Knowledge - 41 Citações

Scopus - 0 Citações

Outras Informações

ID Authenticus: P-00M-Q6W

DOI: 10.1021/acs.bioconjchem.6b00705

Abstract (EN): Several strategies have been implemented to enhance brain drug delivery, and herein solid lipid nanoparticles functionalized with apolipoprotein E were tested in hCMEC/D3 cell monolayers. The mean diameter of 160 nm, negative charge of -12 mV, and their lipophilic characteristics make these nanosystems suitable for brain delivery. Confocal images and flow cytometry data showed a cellular uptake increase of 1.8-fold for SLN-Palmitate-ApoE and 1.9-fold for SLN-DSPE-ApoE when compared with the non-functionalized SLNs. Clathrin-mediated endocytosis was distinguished as the preferential internalization pathway involved in cellular uptake and nanoparticles could cross the blood-brain barrier predominantly by a transcellular pathway. The understanding of the mechanisms involved in the transport of these nanosystems through the blood brain barrier may potentiate their application on brain drug delivery.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10