DEVELOPMENT OF AN HPLC ASSAY METHODOLOGY FOR A DESONIDE CREAM WITH CHEMOMETRICS ASSISTED OPTIMIZATION

• Tipo: Artigo em Revista Científica Internacional
• Data: 2012

Autores

Sandra B Lopes (Autor) Outra; Sarraguca, JM (Autor) Outra; Prior, JAV (Autor) FFUP; Joao A Lopes (Autor) Outra

Revista

Título: Analytical Letters

Vol. 45

Páginas: 1390-1400

ISSN: 0003-2719

Editora: Taylor & Francis

Indexação

ISI Web of Knowledge - 5 Citações

Scopus - 4 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-002-FHJ

DOI: 10.1080/00032719.2012.675494

Abstract (EN): A new reversed-phase liquid chromatographic method for the separation and simultaneous quantification of desonide, sorbic acid, methylparaben, propyl gallate, and the major degradation product of desonide in a hydrophilic cream was developed with the aid of experimental design, resolution loss functions, and chemometrics methods. A strategy involving a screening phase and a fractional factorial design revealed the most influent chromatographic variables (pH and organic solvent content). The arc tangent resolution function was adopted as the optimization loss function. These variables were further optimized using a central composite design. Multivariate curve resolution and partial least squares regression were tested to optimize the chromatographic run time. The latter revealed to be superior in terms of precision and allowed the validation of a method with a total run time 3 times lower (approximately 8 min). The experimental design and chemometrics models enabled an efficient use of time and resources in predicting the optimum separation conditions for the desonide formulation. The validation of the resulting method according to the current ICH guidelines confirmed its selectivity, linearity, accuracy, and precision.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11