The determination and distribution of nucleotides in dairy products using HPLC and diode array detection

• Tipo: Artigo em Revista Científica Internacional
• Data: 2001

Autores

Ferreira, IMPLVO (Autor) FFUP; Mendes, E (Autor) Outra; Gomes, AMP (Autor) Outra; Faria, MA (Autor) Outra; Ferreira, MA (Autor) Outra

Revista

Título: Food Chemistry

Vol. 74

Páginas: 239-244

ISSN: 0308-8146

Editora: Elsevier

Indexação

ISI Web of Knowledge - 36 Citações

Scopus - 29 Citações

Classificação Científica

FOS: Ciências agrárias > Outras ciências agrárias

Outras Informações

ID Authenticus: P-000-TPR

DOI: 10.1016/s0308-8146(01)00133-9

Abstract (EN): A method for the determination of adenosine, cytidine, uridine, guanosine and inosine 5'-monophosphates in milk and dairy products was optimized and its performance evaluated. The technique was based on ion-pair reversed-phase HPLC separation of the nucleotides and diode array detection. The chromatographic separation was achieved using a C18 column and a gradient elution with a mixture of two solvents: solvent A, water/glacial acetic acid/tetrabutilammonium hydrogensulphate (TBAHS) and solvent B, methanol/glacial acetic acid/TBAHS. The effluent was monitored using a Diode Array detector set at 260 nm. Validation of the proposed method was carried out by standard additions method, with recoveries of 98.3%. The precision of the method was also evaluated and reported a coefficient of variation (CV) as less than 3.2. Upon development the technique was applied on different dairy products in order to study the distribution of nucleotides therein. The samples included bovine, ovine and caprine milks, the corresponding manually manufactured cheeses, whey cheese and whey. Three commercial cheeses made from bovine, ovine and caprine milks, respectively, and 20 infant formulae were also analysed. In contrast to their absence in cheese upon preparation nucleotides were present in cheese at the end of ripening; this observation led to the extraction of DNA in order to evaluate whether nucleotides were released from degradation of nucleoproteins.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6