Palmitic Acid and Ergosta-7,22-dien-3-ol Contribute to the Apoptotic Effect and Cell Cycle Arrest of an Extract from Marthasterias glacialis L. in Neuroblastoma Cells

• Tipo: Artigo em Revista Científica Internacional
• Data: 2014

Autores

David M Pereira (Autor) FFUP; Georgina Correia da Silva (Autor) FFUP; Patricia Valentao (Autor) FFUP; Natercia Teixeira (Autor) FFUP; Paula B Andrade (Autor) FFUP

Revista

Título: Marine Drugs

Vol. 12

Páginas: 54-68

ISSN: 1660-3397

Editora: MDPI

Indexação

ISI Web of Knowledge - 23 Citações

Scopus - 33 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina básica

Outras Informações

ID Authenticus: P-009-3G7

DOI: 10.3390/md12010054

Abstract (EN): We describe the effect of a chemically characterized lipophilic extract obtained from Marthasterias glacialis L. against human breast cancer (MCF-7) and human neuroblastoma (SH-SY5Y) cell lines. Evaluation of DNA synthesis revealed that both cell lines were markedly affected in a concentration-dependent way, the SH-SY5Y cell line being more susceptible. Cell cycle arrest was observed, an effect induced by the sterol, ergosta-7,22-dien-3-ol, present in the extract. Morphological evaluation of treated cells showed the advent of lipid droplets and chromatin condensation compatible with apoptosis, which was confirmed by the evaluation of caspase-3 and -9 activities. Palmitic acid was the main compound responsible for this apoptotic effect by a ceramide-independent mechanism that involved endoplasmic reticulum (ER)-stress with upregulation of CCAAT/-enhancer-binding protein homologous protein (CHOP).

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 15