Firefly luciferase inhibition

• Tipo: Outra Publicação em Revista Científica Internacional
• Data: 2010

Autores

Joao M M Leitao (Autor) Outra; Joaquim C G E Esteves da Silva (Autor) FCUP

Revista

Título: Journal of Photochemistry and Photobiology B: Biology

Vol. 101 Nº 1

Páginas: 1-8

ISSN: 1011-1344

Editora: Elsevier

Indexação

ISI Web of Knowledge - 88 Citações

Scopus - 96 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-003-1YM

DOI: 10.1016/j.jphotobiol.2010.06.015

Abstract (EN): Firefly luciferase (Luc) is the most studied of the luciferase enzymes and the mechanism and kinetics of the reactions catalyzed by this enzyme have been relatively well characterized. Luc catalyzes the bioluminescent reaction involving firefly luciferin (D-LH(2)), adenosine triphosphate (ATP), magnesium ion and molecular oxygen with the formation of an electronically excited species (oxyluciferin), inorganic pyrophosphate (PPi), carbon dioxide and adenosine monophosphate (AMP). Luc also catalyzes other non-luminescent reactions, which can interfere with the light production mechanism. Following electronic relaxation, the excited oxyluciferin emits radiation in the visible region of the electromagnetic spectrum (550-570 nm). Among the various possible compounds, several classes of inhibitory substances interfere with the activity of this enzyme: here, we consider substrate-related compounds, intermediates or products of the Luc catalyzed reactions, in addition to anesthetics and, fatty acids. This review summarizes the main inhibitors of Luc and the corresponding inhibition kinetic parameters.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8