Neuroprotective effects on microglia and insights into the structure-activity relationship of an antioxidant peptide isolated from Pelophylax perezi

• Tipo: Artigo em Revista Científica Internacional
• Data: 2022

Autores

Placido, A (Autor) Outra; do Amaral, CP (Autor) Outra; Catia Teixeira (Autor) FCUP; Nogueira, A (Autor) Outra; Brango Vanegas, J (Autor) Outra; Barbosa, EA (Autor) Outra; Moreira, DC (Autor) Outra; Silva Carvalho, AE (Autor) Outra; da Silva, MD (Autor) Outra; Dias, JD (Autor) Outra; Albuquerque, P (Autor) Outra; Saldanha Araujo, F (Autor) Outra; Lima, FCDA (Autor) Outra; Batagin Neto, A (Autor) Outra; Kuckelhaus, S (Autor) Outra; Bessa, LJ (Autor) Outra; Freitas, J (Autor) Outra; Brand, GD (Autor) Outra; Santos, NC (Autor) Outra; Relvas, JB (Autor) Outra; Paula Gomes (Autor) FCUP; Leite, JRSA (Autor) Outra; Eaton, P (Autor) Outra

Revista

Título: Journal Of Cellular And Molecular Medicine

Vol. 26

Páginas: 2793-2807

ISSN: 1582-1838

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 12 Citações

Scopus - 14 Citações

Outras Informações

ID Authenticus: P-00W-F5C

DOI: 10.1111/jcmm.17292

Abstract (EN): Tryptophyllins constitute a heterogeneous group of peptides that are one of the first classes of peptides identified from amphibian's skin secretions. Here, we report the structural characterization and antioxidant properties of a novel tryptophyllin-like peptide, named PpT-2, isolated from the Iberian green frog Pelophylax perezi. The skin secretion of P. perezi was obtained by electrical stimulation and fractionated using RP-HPLC. De novo peptide sequencing was conducted using MALDI MS/MS. The primary structure of PpT-2 (FPWLLS-NH2) was confirmed by Edman degradation and subsequently investigated using in silico tools. PpT-2 shared physicochemical properties with other well-known antioxidants. To test PpT-2 for antioxidant activity in vitro, the peptide was synthesized by solid phase and assessed in the chemical-based ABTS and DPPH scavenging assays. Then, a flow cytometry experiment was conducted to assess PpT-2 antioxidant activity in oxidatively challenged murine microglial cells. As predicted by the in silico analyses, PpT-2 scavenged free radicals in vitro and suppressed the generation of reactive species in PMA-stimulated BV-2 microglia cells. We further explored possible bioactivities of PpT-2 against prostate cancer cells and bacteria, against which the peptide exerted a moderate antiproliferative effect and negligible antimicrobial activity. The biocompatibility of PpT-2 was evaluated in cytotoxicity assays and in vivo toxicity with Galleria mellonella. No toxicity was detected in cells treated with up to 512 mu g/ml and in G. mellonella treated with up to 40 mg/kg PpT-2. This novel peptide, PpT-2, stands as a promising peptide with potential therapeutic and biotechnological applications, mainly for the treatment/prevention of neurodegenerative disorders.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 15