Code: | MA326 | Acronym: | FCC |
Keywords | |
---|---|
Classification | Keyword |
OFICIAL | Biology and Aquatic Environment |
Active? | Yes |
Responsible unit: | Microscopy |
Course/CS Responsible: | Aquatic Sciences |
Acronym | No. of Students | Study Plan | Curricular Years | Credits UCN | Credits ECTS | Contact hours | Total Time |
---|---|---|---|---|---|---|---|
LCMA | 11 | Official Study Plan | 3 | - | 5 | 49 | 135 |
Abilities to achieve: manipulation of cell cultures; recognition of the differences between primary and prolonged cultures; recognition of the limitations of using cell cultures in research studies.
Skills to achieve: working correctly in a cell culture room; to apply correctly the manipulation of cell cultures to genetic and environmental toxicity studies.Control of cell division; mitosis and meiosis.
Cellular differentiation
DNA repair mechanismsTheoretical:
Principles of cell culture. Adherent cultures and in suspension. Choice and type of substracts. Co-cultures. Cell differentiation markers. Quantification and cell viability tests. Application of cell cultures in studies of characterization and conservation of aquatic species. Biomarkers of genotoxicity. Assessement of DNA damage and chromosome instability. Sensitivity studies on mutagenic agents. Immortalization and preservation of cell lines. Determining factors for effective cryopreservation.
Practical:
Introduction to the laboratorial practice. The cell culture environment. Biorisk and biosafety notions and rules. Asepsis techniques and sterilization of material. Contamination of cell cultures. Basic procedures of primary and prolonged cultures. Cell viability tests. Maintenance of cell lines, subdivision and freezing/defrost. Observation and cell count in inverted microscope.
Designation | Weight (%) |
---|---|
Exame | 50,00 |
Trabalho laboratorial | 50,00 |
Total: | 100,00 |
Designation | Time (hours) |
---|---|
Estudo autónomo | 62,00 |
Frequência das aulas | 49,00 |
Trabalho escrito | 24,00 |
Total: | 135,00 |
Final grade of 20 values, distributed in two components: practical report (50%) and final exam (50%)