Molecular BioInterfaces

Code:

L.BIO042

Acronym:

BM

Keywords
Classification	Keyword
OFICIAL	Molecular Bioengineering

Instance: 2023/2024 - 1S (of 11-09-2023 to 15-12-2023)

Active?	Yes
Responsible unit:	Molecular Biology
Course/CS Responsible:	Bachelor in Bioengineering

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
L.BIO	30	Syllabus	3	-	6	65	162

Teaching language

Suitable for English-speaking students

Objectives

Provide the fundamental concepts underlying the interactions at biological interfaces involving the extracellular matrix, biomaterials, cells, proteins, bacteria and blood. Understand how the surface properties of biomaterials, including the chemical composition, topography and mechanical properties, dictate protein adsorption and cellular response. Recognize the importance of the modification and characterization of biomaterial surface properties in order to control its interaction with proteins and cells.

Learning outcomes and competences

1. Understand the principles and concepts involved in the interaction between natural/artificial surfaces and the biological environment.

2. Ability to integrate knowledge on the design, modification and characterization of biomaterial surfaces capable of inducing specific biological responses for a target application;

3. Critical analysis of published scientific data through the presentation and discussion of research papers.

4. Acquire skills in the preparation, modification and characterization of biomaterial surfaces in laboratory classes.

Working method

Presencial

Program

1. Basic aspects on extracellular matrix, cells, proteins and biomaterials interactions.

2. Surface properties of biomaterials: modification strategies.

3. Biointerfaces for cell modulation: cell–substrate and human body-biomaterial interactions.

4. Biointerfaces to control protein, blood and bacteria interactions.

5. Probing and characterization of biointerfaces.

Mandatory literature

Hans-Jürgen Butt, Karlheinz Graf, Michael Kappl; Physics and chemistry of interfaces (Third Edition), Wiley, 2013. ISBN: 9783527412167
William R. Wagner, Shelly E. Sakiyama-Elbert, Guigen Zhang, Michael J. Yaszemski.; Biomaterials Science: An Introduction to Materials in Medicine (Fourth Edition), Academic Press, 2020. ISBN: 9780128161371
Mauli Agrawal, Joo L. Ong, Mark R. Appleford, Gopinath Mani; Introduction to Biomaterials Basic Theory with Engineering Applications, Cambridge University Press, 2014. ISBN: 9780521116909
Martins MCL, Sousa SR, Antunes JC, Barbosa MA; Protein Adsorption Characterization, In Methods in Molecular Biology, “Nanotechnology in Regenerative Medicine: Methods and Protocols” edited by Josep A. Planell and Melba Navarro., Springer Science, 2012. ISBN: 9781617793882
Felgueiras HP, Antunes JC, Martins MCL, Barbosa MA; Fundamentals of protein and cell interactions in biomaterials, In “Peptides and Proteins as Biomaterials for Tissue Regeneration and Repair” edited by Martins MCL, Barbosa MA., Elsevier, 2017. ISBN: 9780081008034

Complementary Bibliography

Gerald H. Pollack. ; Cells, Gels and the Engines of Life, Ehbner and Sons Publishers, 2001. ISBN: 9780962689529
Kay C. Dee, David A. Puleo, Rena Bizios; An Introduction to Tissue-Biomaterial Interactions, John Wiley & Sons, 2002. ISBN: 9780471270591
David A. Puleo, Rena Bizios; Biological Interactions on Materials Surfaces. Understanding and controlling protein, cell and tissue response, Springer Science, 2009. ISBN: 9780387981611
Paul Ducheyne; Comprehensive Biomaterials, Elsevier, 2011. ISBN: 9780080552941

Teaching methods and learning activities

1. Classes covering the fundamental concepts of each topic by the teachers and discussion with the students.

2. Presentation and discussion of research papers by the students.

3. Laboratory classes comprising relevant experiments covering the topics discussed by the teachers.

Evaluation Type

Distributed evaluation with final exam

Assessment Components

Designation	Weight (%)
Apresentação/discussão de um trabalho científico	10,00
Exame	60,00
Trabalho laboratorial	25,00
Participação presencial	5,00
Total:	100,00

Amount of time allocated to each course unit

Designation	Time (hours)
Apresentação/discussão de um trabalho científico	10,00
Estudo autónomo	87,00
Frequência das aulas	39,00
Trabalho laboratorial	26,00
Total:	162,00

Eligibility for exams

According with the current regulations.

Calculation formula of final grade

Final Exam (60%) + Laboratorial Classes (25%) + Presentation of scientific paper (10%) + Presential participation (5%)