Code: | EBE0125 | Acronym: | EC |
Keywords | |
---|---|
Classification | Keyword |
OFICIAL | Molecular Biotechnology |
Active? | Yes |
Responsible unit: | Molecular Biology |
Course/CS Responsible: | Master in Bioengineering |
Acronym | No. of Students | Study Plan | Curricular Years | Credits UCN | Credits ECTS | Contact hours | Total Time |
---|---|---|---|---|---|---|---|
MIB | 22 | Syllabus | 4 | - | 6 | 56 | 162 |
To acquire knowledge in terms of the fundaments that support the understanding and manipulation of cell behaviour.
To acquire knowledge in the most recent approaches for the modification and modulation of cellular function and detailed coverage of the methodology for improving cellular properties used in the production of biopharmaceuticals, gene and cell therapies and tissue engineering.
To develop the students’ reasoning and problem solving skills regarding cellular engineering as well as for the proposal of new therapeutic strategies in the context of distinct diseases, such as cancer and inflammatory diseases.
During the lectures the students will be encouraged to discuss with the lecturer and the peers the themes under analysis. These will be accompanied by practical laboratory sessions in order to illustrate with practical examples current cellular engineering strategies discussed in the lectures. The teaching-learning process will be centered on the work (autonomous and tutored) of the student and on the discussion of the laboratory work in oral group presentations.
Specifically, in this UC is assumed a teaching methodology that is focused on the acquisition of knowledge that integrates a holistic vision of the current cellular engineering strategies and will allow the extrapolation of the use of the different discussed methodologies to new scenarios.
Students are provided with an introduction to engineering principles and modelling at the cellular and organism levels. Of particular interest are receptor/ligand binding, modulation of cellular activities, genetic engineering and cytomechanics.
The following topics are covered:
- The cell and its microenvironment
- Modulation of cellular activities during homeostasis and disease
- Immunomodulatory therapies
- In vitro approaches of genetic, mRNA and protein engineering
- Cytomechanics and mechanotransduction
- Bioreactors
- In vitro engineered tissues as alternatives to animal tests.
Lab sessions are planned with practical examples that will illustrate the concepts discussed in the lectures. The lab works include:
1) Cell culture on different substrates
2) Evaluation of cell behavior on different substrates
3) Gene expression modulation through siRNAs (sequence design basics and siRNA transfections)
4) Methods of assessment of efficiency of cell transfection
a) General theoretical-practical classes will be based on the presentation of the themes of the course unit, along with the discussion of practical examples that illustrate the addressed methodologies and strategies;
b) Laboratory classes on themes of this course unit and discussion of the laboratory work in oral group presentations.
Designation | Weight (%) |
---|---|
Exame | 70,00 |
Trabalho laboratorial | 25,00 |
Apresentação/discussão de um trabalho científico | 5,00 |
Total: | 100,00 |
Designation | Time (hours) |
---|---|
Apresentação/discussão de um trabalho científico | 3,00 |
Frequência das aulas | 39,00 |
Trabalho laboratorial | 14,00 |
Total: | 56,00 |
Students are required to have an active role and to attend, at least, 75% of the practical classes and obtain a minimum classification of 10 out of 20 in the laboratory component.
Final grade = Exam grade * 0,70+ lab sessions grade * 0,25+ work presentation grade * 0,05
Lab sessions grade = 0,30*Grade of the lab session participation + 0,70 * grade of the results oral discussion.
A minimum of 10 out of 20 in the written exam is required for approval.
As specified by the rules applicable to the Mestrado Integrado em Bioengenharia.