3D-nanostructured Au electrodes for the event-specific detection of MON810 transgenic maize

• Tipo: Artigo em Revista Científica Internacional
• Data: 2015

Autores

Fatima Barroso, MF (Autor) Outra; Maria Freitas (Autor) Outra; Beatriz B P P Oliveira (Autor) FFUP; Noemi de los Santos Alvarez (Autor) Outra; Maria J Jesus Lobo Castanon (Autor) Outra; Cristina Delerue Matos (Autor) Outra

Revista

Título: Talanta

Vol. 134

Páginas: 158-164

ISSN: 0039-9140

Editora: Elsevier

Indexação

ISI Web of Knowledge - 9 Citações

Scopus - 12 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-009-ZDP

DOI: 10.1016/j.talanta.2014.10.017

Abstract (EN): In the present work, the development of a genosensor for the event-specific detection of MON810 transgenic maize is proposed. Taking advantage of nanostructuration, a cost-effective three dimensional electrode was fabricated and a ternary monolayer containing a dithiol, a monothiol and the thiolated capture probe was optimized to minimize the unspecific signals. A sandwich format assay was selected as a way of precluding inefficient hybridization associated with stable secondary target structures. A comparison between the analytical performance of the Au nanostructured electrodes and commercially available screen-printed electrodes highlighted the superior performance of the nanostructured ones. Finally, the genosensor was effectively applied to detect the transgenic sequence in real samples, showing its potential for future quantitative analysis.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7