3D-nanostructured Au electrodes for the event-specific detection of MON810 transgenic maize

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Fatima Barroso, MF (Author) Other; Maria Freitas (Author) Other; Beatriz B P P Oliveira (Author) FFUP; Noemi de los Santos Alvarez (Author) Other; Maria J Jesus Lobo Castanon (Author) Other; Cristina Delerue Matos (Author) Other

Journal

Title: Talanta

Vol. 134

Pages: 158-164

ISSN: 0039-9140

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 9 Citations

Scopus - 12 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-009-ZDP

DOI: 10.1016/j.talanta.2014.10.017

Abstract (EN): In the present work, the development of a genosensor for the event-specific detection of MON810 transgenic maize is proposed. Taking advantage of nanostructuration, a cost-effective three dimensional electrode was fabricated and a ternary monolayer containing a dithiol, a monothiol and the thiolated capture probe was optimized to minimize the unspecific signals. A sandwich format assay was selected as a way of precluding inefficient hybridization associated with stable secondary target structures. A comparison between the analytical performance of the Au nanostructured electrodes and commercially available screen-printed electrodes highlighted the superior performance of the nanostructured ones. Finally, the genosensor was effectively applied to detect the transgenic sequence in real samples, showing its potential for future quantitative analysis.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7