Pursuing type 1 diabetes mellitus and related complications through urinary proteomics

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Caseiro, A (Author) Other; Barros, A (Author) Other; Ferreira, R (Author) Other; Padrao, A (Author) Other; Aroso, M (Author) Other; Quintaneiro, C (Author) Other; Pereira, A (Author) Other; Marinheiro, R (Author) Other; Vitorino, R (Author) Other; Amado, F (Author) Other

Journal

The Journal is awaiting validation by the Administrative Services.

Title: TRANSLATIONAL RESEARCH

Vol. 163

Pages: 188-199

ISSN: 1931-5244

Indexing

ISI Web of Knowledge - 34 Citations

Scopus - 33 Citations

Other information

Authenticus ID: P-008-S1Z

DOI: 10.1016/j.trsl.2013.09.005

Abstract (EN): Diabetes mellitus is a chronic metabolic disease with multiple complications, and its successful management requires early diagnosis, to allow timely interventions. Here, we have comprehensively analyzed the proteome changes in urine of type 1 diabetic subjects with and without complications such as retinopathy and nephropathy. gel electrophoresis combined to liquid chromatography-tandem mass spectrometry (GeLC-MS/MS) analysis of midstream urine highlighted the mechanisms involved in disease pathogenesis as, for instance wound healing and blood coagulation in all diabetics or altered ganglioside metabolism in retinopathy, and also some urinary proteins with potential diagnosis value. From these, gelsolin and antithrombin-III appear as promising diagnosis markers for type 1 diabetes mellitus (T1DM), whereas ephrin type-B receptor 4 and vitamin K-dependent protein Z seem to be promising markers for advanced T1DM disease state presenting retinopathy and nephropathy (T1DM-R + N). Data also suggest urinary ganglioside GM2 activator and beta-hexosaminidase subunit beta as potential urinary markers of retinopathy in diabetics. Taken together, the present exploratory urinary proteomic analysis might be seen as an important starting point for studies targeting specific urinary proteins aimed at the implementation of new biomarkers for the early detection of T1DM-related microvascular complications.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12