Primary structure and expression of a nuclear-coded subunit of complex I homologous to proteins specified by the chloroplast genome

• Tipo: Artigo em Revista Científica Internacional
• Data: 1990

Autores

Mário Chaves (Autor) ICBAS

Revista

Título: Biochemical and Biophysical Research Communications

Vol. 171

Páginas: 1168-1174

ISSN: 0006-291X

Editora: Elsevier

Indexação

Scopus - 17 Citações

Classificação Científica

FOS: Ciências exactas e naturais

Outras Informações

ID Authenticus: P-008-NKZ

Abstract (EN): A 31-kDa subunit of complex I from Neurospora crassa, of nuclear origin, was cloned. The precursor polypeptide (33 kDa) could be efficiently expressed in an in vitro system for transcription and translation. The processing of the precursor to the mature protein was also obtained in vitro. An open reading frame coding for a precursor protein of 283 amino acids (32247 Da) was found by DNA sequencing. The predicted primary structure shows significant homology with proteins made in chloroplast. This supports the hypothesis that an enzyme similar to respiratory chain NADH dehydrogenase might exist in these organelles. © 1990.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7