Evaluation of Symmetry Enhanced Sliding Band Filter for Plant Cell Nuclei Detection in Low Contrast Noisy Fluorescent Images

• Tipo: Artigo em Livro de Atas de Conferência Internacional
• Data: 2009

Autores

Monica Marcuzzo (Autor) Outra; Pedro Quelhas (Autor) Outra; Ana Maria Mendonca (Autor) FEUP; Aurelio Campilho (Autor) FEUP

Ata de Conferência Internacional

Título: Image Analysis and Recognition

Páginas: 824-831

6th International Conference, ICIAR 2009

Halifax, Canada, July 6-8, 2009

Indexação

ISI Proceedings

ISI Web of Knowledge - 7 Citações

Scopus - 8 Citações

INSPEC

COMPENDEX

Classificação Científica

FOS: Ciências exactas e naturais > Ciências da computação e da informação

CORDIS: Ciências Tecnológicas > Engenharia > Engenharia biomédica

Outras Informações

ID Authenticus: P-003-QHN

DOI: 10.1007/978-3-642-02611-9_81

Abstract (EN): The study of cell nuclei is fundamental for plant cell Biology research. To obtain information at; cellular level, researchers image cells' nuclei which were modified with fluorescence proteins, through laser scanning confocal microscopy. These images are normally noisy and suffer from high background fluorescence, making grey-scale segmentation approaches inadequate for a usable detection. To obtain a successful detection even at low contrast we investigate the use of a particular convergence filter, the Symmetric Sliding Band filter (SSBF), for cell detection. This filter is based on gradient convergence and not intensity. As such it can detect low contrast cell nuclei which otherwise would be lost in the background noise. Due to the characteristics of cell nuclei morphology, a symmetry constrain is integrated in the filter which corrects some inadequate detections and results in a filter response that is more discriminative. We evaluate the use of this filter for cell nuclei detection on the Arabidopsis thaliana root tip, where the nuclei were stained using yellow fluorescence protein. The resulting cell nuclei detection precision is 89%.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8