Dissemination amongst humans and food products of animal origin of a Salmonella typhimurium clone expressing an integron-borne OXA-30 beta-lactamase

• Tipo: Artigo em Revista Científica Internacional
• Data: 2004

Autores

Antunes, P (Autor) FCNAUP; Machado, J (Autor) Outra; Sousa, JC (Autor) Outra; Peixe, L (Autor) FFUP

Revista

Título: Journal of Antimicrobial Chemotherapy

Vol. 54 Nº 2

Páginas: 429-434

ISSN: 0305-7453

Editora: Oxford University Press

Indexação

ISI Web of Knowledge - 49 Citações

Scopus - 50 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Ciências da saúde

Outras Informações

ID Authenticus: P-000-96E

DOI: 10.1093/jac/dkh333

Abstract (EN): Objectives: Characterization of the molecular basis for beta-lactam resistance and evaluation of the clonal relatedness among nine isolates of multidrug-resistant Salmonella typhimurium recovered from seven clinical human samples and two pork end products. Methods: The isolates were examined for susceptibility to antimicrobial agents. The relationships between resistance genes, class 1 integrons, plasmids and isolates were screened by molecular methods such as polymerase chain reaction and restriction fragment length analysis. Results: A bla(OXA-30) gene, located in a class 1 integron, was detected in all isolates. This integron was present on a conjugative plasmid in all but one isolate. By pulsed-field gel electrophoresis, it was determined that all strains share the same chromosomal type. Conclusions: This study demonstrates the spread of an OXA-30-producing S. typhimurium in Portugal, suggesting dissemination of a resistant clone through the food chain.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6