The effect of a fluorinated cholesterol derivative on the stability and physical properties of cationic DNA vectors

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

Diana Paiva (Autor) FEUP; Albertoo Martín-Molina (Autor) Outra; Isabel Cardoso (Autor) Outra; Manuel Quesada-Pérez (Autor) Outra; Maria do Carmo Pereira (Autor) FEUP; Sandra Rocha (Autor) FEUP

Revista

Título: Soft Matter

Vol. 9 Nº 2

Páginas: 401-409

ISSN: 1744-683X

Editora: Royal Society of Chemistry

Indexação

ISI Web of Knowledge - 14 Citações

ISI Web of Science

Scopus - 14 Citações

Classificação Científica

FOS: Ciências da engenharia e tecnologias > Engenharia dos materiais

CORDIS: Ciências da Saúde ; Ciências Tecnológicas

Projetos Associados

Referência: PTDC/QUI-BIQ/102827/2008 - Concepção de péptidos fluorados para inibição da agregação do péptido beta-amilóide e avaliação da sua actividade biológica

PTDC/QUI-BIQ/118076/2010 - Liposomas funcionalizados com anticorpos para a doença de Alzheimer

Outras Informações

ID Authenticus: P-002-266

DOI: 10.1039/c2sm27236a

Abstract (EN): Liposomes of the cationic lipid DOTAP (1,2-dioleoyl-3-trimethylammonium-propane) and the fluorinated cholesterol derivative heptafluorocholesterol (F7-CHOL) were tested at the molar ratio of 1 : 1 for DNA compaction and transfection. Their properties were correlated with the characteristics of the well-known system, DOTAP and cholesterol (CHOL). The mass lipid-DNA (L/D) ratios at the isoelectric point were within the ranges 3-4 for DOTAP: CHOL and 4-5 for DOTAP: F7-CHOL, as determined by electrophoretic mobility measurements. These results and the ethidium bromide fluorescence intercalation assays confirmed that more DOTAP: F7-CHOL liposomes are needed to compact the same amount of DNA as DOTAP: CHOL. The phase diagrams of aggregation and re-entrant condensation phenomena obtained by phenomenological theory support this conclusion and also establish that the liposome-DNA binding is stronger in the case of the DOTAP:F7-CHOL-DNA system. The stability rates of both liposomes in the presence of DNA and their transfection efficiencies were similar, which support the application of heptafluorocholesterol as a helper lipid in cationic liposomes for DNA delivery.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9