Colorimetric detection of eukaryotic gene expression with DNA-derivatized gold nanoparticles

• Tipo: Artigo em Revista Científica Internacional
• Data: 2005

Autores

Baptista, P (Autor) Outra; Doria, G (Autor) Outra; Henriques, D (Autor) Outra; Pereira, E (Autor) FCUP; Franco, R (Autor) Outra

Revista

Título: Journal of Biotechnology

Vol. 119

Páginas: 111-117

ISSN: 0168-1656

Editora: Elsevier

Indexação

ISI Web of Knowledge - 80 Citações

Scopus - 64 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-000-1AH

DOI: 10.1016/j.jbiotec.2005.02.019

Abstract (EN): Thiol-linked DNA-gold nanoparticles were used in a novel colorimetric method to detect the presence of specific mRNA from a total RNA extract of yeast cells. The method allowed detection of expression of the FSY1 gene that encodes a specific fructose/H+ symporter in Saccharomyces bayanus PYCC 4565. FSY1 is strongly expressed when the yeast is grown in fructose as the sole carbon source, while cells cultivated in glucose as the sole carbon source repress gene expression. The presence of FSY1 mRNA is detected based on color change of a sample containing total RNA extracted from the organism and gold nanoparticles derivatized with a 15-mer of complementary single stranded DNA upon addition of NaCl. If FSY1 mRNA is present, the solution remains pink, changing to blue-purple in the absence of FSY1 mRNA. Direct detection of specific expression was possible from only 0.3 mu g of unamplified total RNA without any further enhancement. This novel method is inexpensive, very easy to perform as no amplification or signal enhancement steps are necessary and takes less than 15 min to develop after total RNA extraction. No temperature control is necessary and color change can be easily detected visually.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7