Antibiofilm Effect of Nitric Acid-Functionalized Carbon Nanotube-Based Surfaces against <i>E. coli</i> and <i>S. aureus</i>

• Tipo: Artigo em Revista Científica Internacional
• Data: 2023

Autores

Rita Santos (Autor) FEUP; Marisa Gomes (Autor) Outra; Luciana Gomes (Autor) FEUP; Francisca Cardoso (Autor) FEUP; Fábio Carvalho (Autor) Outra; Andreia Tomé (Autor) FEUP; O.S.G.P. Soares (Autor) FEUP; Kathryn Whitehead (Autor) Outra; Filipe Mergulhão (Autor) FEUP

Revista

Título: Antibiotics

Vol. 12

Página Final: 1620

Editora: MDPI

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 0 Citações

Outras Informações

ID Authenticus: P-00Z-AN8

DOI: 10.3390/antibiotics12111620

Abstract (EN): Chemically modified carbon nanotubes are recognized as effective materials for tackling bacterial infections. In this study, pristine multi-walled carbon nanotubes (p-MWCNTs) were functionalized with nitric acid (f-MWCNTs), followed by thermal treatment at 600 C-degrees, and incorporated into a poly(dimethylsiloxane) (PDMS) matrix. The materials' textural properties were evaluated, and the roughness and morphology of MWCNT/PDMS composites were assessed using optical profilometry and scanning electron microscopy, respectively. The antibiofilm activity of MWCNT/PDMS surfaces was determined by quantifying culturable Escherichia coli and Staphylococcus aureus after 24 h of biofilm formation. Additionally, the antibacterial mechanisms of MWCNT materials were identified by flow cytometry, and the cytotoxicity of MWCNT/PDMS composites was tested against human kidney (HK-2) cells. The results revealed that the antimicrobial activity of MWCNTs incorporated into a PDMS matrix can be efficiently tailored through nitric acid functionalization, and it can be increased by up to 49% in the absence of surface carboxylic groups in f-MWCNT samples heated at 600 C-degrees and the presence of redox activity of carbonyl groups. MWCNT materials changed the membrane permeability of both Gram-negative and Gram-positive bacteria, while they only induced the production of ROS in Gram-positive bacteria. Furthermore, the synthesized composites did not impact HK-2 cell viability, confirming the biocompatibility of MWCNT composites.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 16