Separation of racemic chiral drugs using immobilized CHIRALPAK IA: methodology for preparative scale development

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

Miriam Zabkova (Autor) FEUP; Michal Zabka (Autor) FEUP; Alírio E. Rodrigues (Autor) FEUP

Revista

Título: Separation Science and Technology

Páginas: 275-303

ISSN: 0149-6395

Editora: Taylor & Francis

Indexação

ISI Web of Knowledge - 10 Citações

ISI Web of Science

Scopus - 11 Citações

COMPENDEX

Classificação Científica

FOS: Ciências da engenharia e tecnologias > Engenharia química

CORDIS: Ciências Tecnológicas > Engenharia > Engenharia química

Outras Informações

ID Authenticus: P-003-S4J

DOI: 10.1080/01496390802590079

Abstract (EN): Immobilized stationary phases represent a new perspective for analytical as well as preparative scale separation of chiral species. In this study, the basic data necessary for further development of the production-scale processes using CHIRALPAK IA columns were acquired. The new immobilized chiral stationary phase CHIRALPAK IA has proven to be useful for the chiral separations of propranolol, metoprolol, guaifenesin, and -Tetralol enantiomers. A broad range of standard and non-standard solvents used as components of mobile phases were tested and their influence on the separations was evaluated. A satisfactory separation considering further utilization of simulated moving bed chromatography was found for propranolol, guaifenesin, and -tetralol using the following mobile phases n-Heptane/methanol/ethanolamine (05/95/0.1, v/v/v), n-Heptane/ethanol (85/15, v/v) and n-Heptane/DCM (85/15, v/v), respectively. Inverse size exclusion chromatography was implemented to measure the porosity of the column using the set of polystyrene standards. The multicomponent adsorption equilibrium was characterized by linear + Langmuir model for -Tetralol and linear model for propranolol and guaifenesin due to their limited solubility in selected mobile phases. The isotherm model was successfully validated by comparison of pulse profiles obtained by solving the mathematical model and those obtained experimentally.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 29