Comparative study of screening methodologies for ochratoxin A detection in winery by-products

• Tipo: Artigo em Revista Científica Internacional
• Data: 2008

Autores

Ribeiro, E (Autor) Outra; Alves, A (Autor) FEUP

Revista

Título: Analytical and Bioanalytical Chemistry

Vol. 391 Nº 4

Páginas: 1443-1450

ISSN: 1618-2642

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 4 Citações

ISI Web of Science

Scopus - 10 Citações

COMPENDEX

Classificação Científica

CORDIS: Ciências Físicas > Química > Química analítica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-003-YMJ

DOI: 10.1007/s00216-008-1861-y

Abstract (EN): The worldwide contamination of winery by-products by mycotoxins may present a serious hazard to human and animal health. Mycotoxins are secondary metabolites of fungi with possible adverse effects on humans, animals, and crops that result in illnesses and economic losses. Mycotoxins are under continuous survey in Europe, but the regulatory aspects still need to be set up for winery by-products, which may be used in animal feed. The aim of this study was to implement a simple but reliable analytical methodology for ochratoxin A (OTA) quantification in grape pomaces in order to perform a survey of samples from the Douro Demarcated Region, Portugal. The method involved a unique preparation step, solvent extraction, followed by high-performance liquid chromatography (HPLC) with fluorescence (FL) detection. A comparative study was performed with two extraction solvents (ethyl acetate and methanol) as well as using extraction on an immunoaffinity column. The linearity range for OTA analysis was 0.05-23.5 mu g L(-1) with a detection limit of 0.05 mu g L(-1) and a precision (expressed by the coefficient of variation under repeatability conditions) of 0.4-14.7%. The percentage of recovery was on average 23.5 +/- 3.6% (extraction with ethyl acetate) or 70.1 +/- 2.5% (extraction with 70% methanol). Accounting for the recovery factor and the chromatographic detection limit, as well as the preconcentration factor, the limit of detection in grape pomaces is 0.04 mu g kg(-1) (ethyl acetate extraction) and 0.33 mu g kg(-1) (methanol extraction). Samples from 12 out of 13 sites in the Douro Demarcated Region showed OTA presence with concentrations not exceeding 0.4 mu g kg(-1). Both developed methods for evaluation of OTA in grape pomace are simple but efficient.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8