Discovery of Cyanobacterial Natural Products Containing Fatty Acid Residues**

• Tipo: Artigo em Revista Científica Internacional
• Data: 2021

Autores

Figueiredo, SAC (Autor) Outra; Preto, M (Autor) Outra; Moreira, G (Autor) Outra; Martins, TP (Autor) Outra; Abt, K (Autor) Outra; Andre Melo (Autor) FCUP; Vitor Vasconcelos (Autor) FCUP; Pedro N Leao (Autor) Outra

Revista

Título: Angewandte Chemie - International Edition

Vol. 60

ISSN: 1433-7851

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 11 Citações

Scopus - 12 Citações

Outras Informações

ID Authenticus: P-00T-P6M

DOI: 10.1002/anie.202015105

Abstract (EN): In recent years, extensive sequencing and annotation of bacterial genomes has revealed an unexpectedly large number of secondary metabolite biosynthetic gene clusters whose products are yet to be discovered. For example, cyanobacterial genomes contain a variety of gene clusters that likely incorporate fatty acid derived moieties, but for most cases we lack the knowledge and tools to effectively predict or detect the encoded natural products. Here, we exploit the apparent absence of a functional beta-oxidation pathway in cyanobacteria to achieve efficient stable-isotope-labeling of their fatty acid derived lipidome. We show that supplementation of cyanobacterial cultures with deuterated fatty acids can be used to easily detect natural product signatures in individual strains. The utility of this strategy is demonstrated in two cultured cyanobacteria by uncovering analogues of the multidrug-resistance reverting hapalosin, and novel, cytotoxic, lactylate-nocuolin A hybrids-the nocuolactylates.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9