3D chitosan-gelatin-chondroitin porous scaffold improves osteogenic differentiation of mesenchymal stem cells

• Tipo: Artigo em Revista Científica Internacional
• Data: 2007

Autores

Machado, CB (Autor) Outra; Ventura, JMG (Autor) Outra; Lemos, AF (Autor) Outra; Ferreira, JMF (Autor) Outra; Leite, MF (Autor) Outra; Goes, AM (Autor) Outra

Revista

Título: Biomedical Materials (Bristol)

Vol. 2

Páginas: 124-131

ISSN: 1748-6041

Editora: Institute of Physics Publishing

Indexação

ISI Web of Knowledge - 38 Citações

Scopus - 38 Citações

Outras Informações

ID Authenticus: P-004-9J0

DOI: 10.1088/1748-6041/2/2/010

Abstract (EN): A porous 3D scaffold was developed to support and enhance the differentiation process of mesenchymal stem cells (MSC) into osteoblasts in vitro. The 3D scaffold was made with chitosan, gelatin and chondroitin and it was crosslinked by EDAC. The scaffold physicochemical properties were evaluated. SEM revealed the high porosity and interconnection of pores in the scaffold; rheological measurements show that the scaffold exhibits a characteristic behavior of strong gels. The elastic modulus found in compressive tests of the crosslinked scaffold was about 50 times higher than the non-crosslinked one. After 21 days, the 3D matrix submitted to hydrolytic degradation loses above 40% of its weight. MSC were collected from rat bone marrow and seeded in chitosan-gelatin-chondroitin 3D scaffolds and in 2D culture plates as well. MSC were differentiated into osteoblasts for 21 days. Cell proliferation and alkaline phosphatase activity were followed weekly during the osteogenic process. The osteogenic differentiation of MSC was improved in 3D culture as shown by MTT assay and alkaline phosphatase activity. On the 21st day, bone markers, osteopontin and osteocalcin, were detected by the PCR analysis. This study shows that the chitosan-gelatin-chondroitin 3D structure provides a good environment for the osteogenic process and enhances cellular proliferation. © 2007 IOP Publishing Ltd.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8