Quercetin lipid nanoparticles functionalized with transferrin for Alzheimer's disease

• Tipo: Artigo em Revista Científica Internacional
• Data: 2020

Autores

R. G. R. Pinheiro (Autor) Outra; Andreia Granja (Autor) Outra; Joana A. Loureiro (Autor) FEUP; Maria do Carmo Pereira (Autor) FEUP; Marina Pinheiro (Autor) Outra; Ana Rute Neves (Autor) Outra; Salette Reis (Autor) Outra

Revista

Título: European Journal of Pharmaceutical Sciences

Vol. 148

Páginas: 1-11

ISSN: 0928-0987

Editora: Elsevier

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 17 Citações

Outras Informações

ID Authenticus: P-00S-1MB

DOI: 10.1016/j.ejps.2020.105314

Abstract (EN): Quercetin was encapsulated in lipid nanoparticles (SLN and NLC) to take advantage of its neuroprotective properties in Alzheimer's disease. The nanoparticles were functionalized with transferrin to facilitate the passage across the blood-brain barrier through the transferrin receptors overexpressed in brain endothelial cells. NMR and FTIR confirmed the functionalization of the nanoparticles with transferrin. TEM results showed all nanoparticles presented spherical morphology. Nanoparticles exhibited size around 200 nm and zeta potential values higher than -30 mV. Quercetin entrapment efficiency was around 80-90%. LDH cytotoxicity assays in hCMEC/D3 cell line demonstrated that even for the highest concentration (30 mu M) nanoparticles did not reveal cytotoxicity after 4 h of incubation. Permeability studies across hCMEC/D3 cell monolayers showed NLC permeate more the blood-brain barrier, while amyloid-beta studies demonstrated NLC-transferrin have the capacity to inhibit fibril formation. Nanoparticles seem to be suitable for brain applications, mainly for Alzheimer's disease due to inhibition of amyloid-beta aggregation.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11