Factorial design as a tool for the optimization of plga nanoparticles for the co-delivery of temozolomide and o6-benzylguanine

• Tipo: Artigo em Revista Científica Internacional
• Data: 2019

Autores

Maria João Ramalho (Autor) FEUP; Joana A. Loureiro (Autor) FEUP; Manuel A. N. Coelho (Autor) FEUP; Maria do Carmo Pereira (Autor) FEUP

Revista

Título: Pharmaceutics

Vol. 11 Nº 8

Páginas: 1-18

ISSN: 1999-4923

Editora: MDPI

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 8 Citações

Projetos Associados

LEPABE-2-ECO-INNOVATION - LEPABE-2-ECO-INNOVATION

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

LEPABE - Projeto Estratégico do LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Outras Informações

ID Authenticus: P-00R-1CV

DOI: 10.3390/pharmaceutics11080401

Abstract (EN): Poly(D,L-lactic-co-glycolic) (PLGA) nanoparticles (NPs) have been widely studied for several applications due to their advantageous properties, such as biocompatibility and biodegradability. Therefore, these nanocarriers could be a suitable approach for glioblastoma multiforme (GBM) therapy. The treatment of this type of tumours remains a challenge due to intrinsic resistance mechanisms. Thus, new approaches must be envisaged to target GBM tumour cells potentially providing an efficient treatment. Co-delivery of temozolomide (TMZ) and O6-benzylguanine (O6BG), an inhibitor of DNA repair, could provide good therapeutic outcomes. In this work, a fractional factorial design (FFD) was employed to produce an optimal PLGA-based nanoformulation for the co-loading of both molecules, using a reduced number of observations. The developed NPs exhibited optimal physicochemical properties for brain delivery (dimensions below 200 nm and negative zeta potential), high encapsulation efficiencies (EE) for both drugs, and showed a sustained drug release for several days. Therefore, the use of an FFD allowed for the development of a nanoformulation with optimal properties for the co-delivery of TMZ and O6BG to the brain.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 18