Revealing cardiolipins influence in the construction of a significant mitochondrial membrane model

• Tipo: Artigo em Revista Científica Internacional
• Data: 2018

Autores

Lopes, SC (Autor) Outra; Ivanova, G (Autor) Outra; de Castro, B (Autor) FCUP; Paula Gameiro (Autor) FCUP

Revista

Título: Biochimica et Biophysica Acta: Biomembranes

Vol. 1860

Páginas: 2465-2477

ISSN: 0005-2736

Editora: Elsevier

Indexação

ISI Web of Knowledge - 4 Citações

Scopus - 4 Citações

Outras Informações

ID Authenticus: P-00P-GX4

DOI: 10.1016/j.bbamem.2018.07.006

Abstract (EN): Cardiolipins are essential for the integrity and the dynamics of the mitochondria membrane, where they exclusively exist in eukaryotes. Changes in cardiolipins membrane levels have been related to several cardiac health disorders. To evaluate cardiolipins impact on membrane properties a physico-chemical study was conducted using steady-state fluorescence anisotropy, dynamic light scattering and Nuclear Magnetic Resonance (H-1 and P-31, NMR). Different binary and ternary mixtures of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine and a natural extract of bovine heart cardiolipin were used as models of mitochondrial membrane. The main transition temperatures, obtained by the first two techniques, revealed to be cardiolipins dependent. Cardiolipins also showed to act as a bidirectional regulator of membrane fluidity. H-1 and P-31 NMR results revealed that cardiolipins affects the conformation, mobility and structural order of the phospholipid molecules. According to H-1 NMR results, cardiolipins disturbs the overall structure and packing order of membrane demonstrated with the decrease of the line broadening and shift of all resonances. The P-31 NMR line shape analysis confirmed that, at distinct temperatures, different lipid phases coexist in the systems, and their type and quantitative distribution are cardiolipins dependent. In summary, cardiolipins presence/absence dramatically changes the membrane properties and has a major impact in the construction of a mitochondrial membrane model.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 13