Resveratrol induces ordered domains formation in biomembranes: Implication for its pleiotropic action

• Tipo: Artigo em Revista Científica Internacional
• Data: 2016

Autores

Neves, AR (Autor) Outra; Nunes, C (Autor) Outra; Salette Reis (Autor) FFUP

Revista

Título: Biochimica et Biophysica Acta: Biomembranes

Vol. 1858

Páginas: 12-18

ISSN: 0005-2736

Editora: Elsevier

Indexação

ISI Web of Knowledge - 11 Citações

Scopus - 21 Citações

Outras Informações

ID Authenticus: P-00J-YV5

DOI: 10.1016/j.bbamem.2015.10.005

Abstract (EN): Resveratrol is a polyphenol compound with great value in cancer therapy, cardiovascular protection, and neuro-degenerative disorders. The mechanism by which resveratrol exerts such pleiotropic effects is not yet clear and there is a huge need to understand the influence of this compound on the regulation of lipid domains formation on membrane structure. The aim of the present study was to reveal potential molecular interactions between resveratrol and lipid rafts found in cell membranes by means of Forster resonance energy transfer, DPH fluorescence quenching, and triton X-100 detergent resistance assay. Liposomes composed of egg phosphatidylcholine, cholesterol, and sphingomyelin were used as model membranes. The results revealed that resveratrol induces phase separation and formation of liquid-ordered domains in bilayer structures. The formation of such tightly packed lipid rafts is important for different signal transduction pathways, through the regulation of membrane-associating proteins, that can justify several pharmacological activities of this compound.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7