Multiple Lipid Nanoparticles (MLN), a New Generation of Lipid Nanoparticles for Drug Delivery Systems: Lamivudine-MLN Experimental Design

• Tipo: Artigo em Revista Científica Internacional
• Data: 2017

Autores

Cavalcanti, SMT (Autor) Outra; Nunes, C (Autor) Outra; Lima, SAC (Autor) Outra; Soares Sobrinho, JL (Autor) Outra; Salette Reis (Autor) FFUP

Revista

Título: Pharmaceutical Research

Vol. 34

Páginas: 1204-1216

ISSN: 0724-8741

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 3 Citações

Scopus - 7 Citações

Outras Informações

ID Authenticus: P-00M-KVB

DOI: 10.1007/s11095-017-2136-0

Abstract (EN): An optimized methodology for the development of a new generation of lipid nanoparticles, the multiple lipid nanoparticles (MLN) is described. MLN have characteristics between nanostructured lipid carriers (NLC) and multiple emulsions (W/O/W), but without the outer aqueous phase. The production is based on a hot homogenization method combined with high shear and ultrasonication. The antiretroviral agent lamivudine (3TC), was loaded in the MLN. For comparison purposes, NLC-3TC formulation was also developed and physico-chemically characterized by the same parameters as MLN-3TC. The development and optimization of MLN and NLC formulations were supported by a Quality by Design (QbD) approach. The MLN-3TC formulation exhibited a size of about 450 nm, polydispersity < 0.3 and negative zeta potential > -20 mV. Furthermore, the morphology assessed by TEM showed a structure with multiples aqueous vacuoles. MLN-3TC was physically stable for at least 45 days, had low cytotoxicity and drug release studies showed a sustained and controlled release of 3TC under gastric and plasma-simulated conditions (at pH 7.4 for about 45 h). The optimized formulations present suitable profiles for oral administration. Overall, the results reveal that MLN present higher loading capacity and storage stability than NLC.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 13