Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach

• Tipo: Artigo em Revista Científica Internacional
• Data: 2015

Autores

Rita C Alves (Autor) Outra; Filipa B Pimentel (Autor) Outra; Henri P A Nouws (Autor) Outra; Wagner Correr (Autor) Outra; Maria Begona Gonzalez Garcia (Autor) Outra; Beatriz B P P Oliveira (Autor) FFUP; Cristina Delerue Matos (Autor) Outra

Revista

Título: Analytical and Bioanalytical Chemistry

Vol. 407

Páginas: 7157-7163

ISSN: 1618-2642

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 16 Citações

Scopus - 31 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-00G-JJJ

DOI: 10.1007/s00216-015-8879-8

Abstract (EN): A voltammetric biosensor for Ara h 6 (a peanut allergen) detection in food samples was developed. Gold nanoparticle-modified screen-printed carbon electrodes were used to develop a sandwich-type immunoassay using two-monoclonal antibodies. The antibody-antigen interaction was detected through the electrochemical detection of enzymatically deposited silver. The immunosensor presented a linear range between 1 and 100 ng/ml, as well as high precision (inter-day RSD a parts per thousand currency sign9.8 %) and accuracy (recoveries a parts per thousand yen96.7 %). The detection and quantification limits were 0.27 and 0.88 ng/ml, respectively. It was possible to detect small levels of Ara h 6 in complex food matrices.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7