Protective effect of C-sativa leaf extract against UV mediated-DNA damage in a human keratinocyte cell line

• Tipo: Artigo em Revista Científica Internacional
• Data: 2015

Autores

Isabel F Almeida (Autor) FFUP; Pinto, AS (Autor) Outra; Monteiro, C (Autor) Outra; Monteiro, H (Autor) Outra; Belo, L (Autor) FFUP; Fernandes, J (Autor) Outra; Bento, AR (Autor) Outra; Duarte, TL (Autor) Outra; Garrido, J (Autor) Outra; M.F. Bahia (Autor) FFUP; José Manuel Sousa Lobo (Autor) FFUP; Costa, PC (Autor) FFUP

Revista

Título: Journal of Photochemistry and Photobiology B: Biology

Vol. 144

Páginas: 28-34

ISSN: 1011-1344

Editora: Elsevier

Indexação

ISI Web of Knowledge - 26 Citações

Scopus - 30 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-00A-9YX

DOI: 10.1016/j.jphotobiol.2015.01.010

Abstract (EN): Toxic effects of ultraviolet (UV) radiation on skin include protein and lipid oxidation, and DNA damage. The latter is known to play a major role in photocarcinogenesis and photoaging. Many plant extracts and natural compounds are emerging as photoprotective agents. Castanea sativa leaf extract is able to scavenge several reactive species that have been associated to UV-induced oxidative stress. The aim of this work was to analyze the protective effect of C. sativa extract (ECS) at different concentrations (0.001, 0.01, 0.05 and 0.1 mu g/mL) against the UV mediated-DNA damage in a human keratinocyte cell line (HaCaT). For this purpose, the cytokinesis-block micronucleus assay was used. Elucidation of the protective mechanism was undertaken regarding UV absorption, influence on O-1(2) mediated effects or NRF2 activation. ECS presented a concentration-dependent protective effect against UV-mediated DNA damage in HaCaT cells. The maximum protection afforded (66.4%) was achieved with the concentration of 0.1 mu g/mL. This effect was found to be related to a direct antioxidant effect (involving O-1(2)) rather than activation of the endogenous antioxidant response coordinated by NRF2. Electrochemical studies showed that the good antioxidant capacity of the ECS can be ascribed to the presence of a pool of different phenolic antioxidants. No genotoxic or phototoxic effects were observed after incubation of HaCaT cells with ECS (up to 0.1 mu g/mL). Taken together these results reinforce the putative application of this plant extract in the prevention/minimization of UV deleterious effects on skin.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7