Rapid automated method for on-site determination of sulfadiazine in fish farming: a stainless steel veterinary syringe coated with a selective membrane of PVC serving as a potentiometric detector in a flow-injection-analysis system

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Almeida, SAA (Autor) Outra; Amorim, LR (Autor) Outra; Heitor, AH (Autor) Outra; Montenegro, MCBSM (Autor) FFUP; Barbosa, J (Autor) Outra; Sa, LC (Autor) Outra; Sales, MGF (Autor) Outra

Revista

Título: Analytical and Bioanalytical Chemistry

Vol. 401

Páginas: 3355-3365

ISSN: 1618-2642

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 8 Citações

Scopus - 10 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-002-JBP

DOI: 10.1007/s00216-011-5441-1

Abstract (EN): Sulfadiazine is an antibiotic of the sulfonamide group and is used as a veterinary drug in fish farming. Monitoring it in the tanks is fundamental to control the applied doses and avoid environmental dissemination. Pursuing this goal, we included a novel potentiometric design in a flow-injection assembly. The electrode body was a stainless steel needle veterinary syringe of 0.8-mm inner diameter. A selective membrane of PVC acted as a sensory surface. Its composition, the length of the electrode, and other flow variables were optimized. The best performance was obtained for sensors of 1.5-cm length and a membrane composition of 33% PVC, 66% o-nitrophenyloctyl ether, 1% ion exchanger, and a small amount of a cationic additive. It exhibited Nernstian slopes of 61.0 mV decade(-1) down to 1.0 x 10(-5) mol L(-1), with a limit of detection of 3.1 x 10(-6) mol L(-1) in flowing media. All necessary pH/ionic strength adjustments were performed online by merging the sample plug with a buffer carrier of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, pH 4.9. The sensor exhibited the advantages of a fast response time (less than 15 s), long operational lifetime (60 days), and good selectivity for chloride, nitrite, acetate, tartrate, citrate, and ascorbate. The flow setup was successfully applied to the analysis of aquaculture waters. The analytical results were validated against those obtained with liquid chromatography-tandem mass spectrometry procedures. The sampling rate was about 84 samples per hour and recoveries ranged from 95.9 to 106.9%.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11