Genotypic analysis of Candida albicans isolates obtained from removable prosthesis wearers

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Costa, F (Author) Other; Manaia, CM (Author) Other; Figueiral, MH (Author) FMDUP; Pinto, E (Author) FFUP

Journal

Title: Letters in Applied Microbiology

Vol. 46 No. 4

Pages: 445-449

ISSN: 0266-8254

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 8 Citations

ISI Web of Science

Scopus - 12 Citations

Pubmed / Medline

Scientific classification

FOS: Natural sciences > Biological sciences

CORDIS: Health sciences

Other information

Authenticus ID: P-004-0MP

DOI: 10.1111/j.1472-765x.2008.02336.x

Abstract (EN): Aims: To assess of the genotypic diversity of Candida albicans isolated from removable prosthesis wearers, with and without denture-related stomatitis (DRS). The occurrence of different genotypes in pathological and control cases was investigated. Methods and Results: One hundred and sixty-four isolates of C. albicans obtained from different oral cavity locations were compared by randomly amplified polymorphic DNA (RAPD). The coherence of this analysis was confirmed by genotyping a selected group of isolates with pulsed field gel electrophoresis (PFGE). Among the 164 isolates, 150 were grouped into seven groups on the basis of their RAPD patterns. Three of these groups (comprising 54 isolates) had significant (alpha < 0.10) predominance of clinical or control cases. For the other isolates, no significant differences were observed between control and DRS cases. Occasionally, more than one genotype was found in the same person. These findings were sustained by PFGE analysis. No relevant associations between the genotypic patterns and pathology level were found. Conclusions: This study evidenced that C. albicans with similar genotypes may be found in individuals with DRS and in control cases. Significance and Impact of the Study: This conclusion hints the involvement of other aetiological factors that alone or in association with C. albicans may trigger the emergence of DRS.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5