Single determination of alpha-ketoglutaric acid and pyruvic acid in beer by HPLC with UV detection

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Patricia Montenegro (Author) Other; Ines Maria Valente (Author) Other; Luis Moreira Goncalves (Author) Other; Jose Antonio Rodrigues (Author) FCUP; Aquiles Araujo Barros (Author) FCUP

Journal

Title: Analytical Methods

Vol. 3

Pages: 1207-1212

ISSN: 1759-9660

Publisher: Royal Society of Chemistry

Indexing

ISI Web of Knowledge - 21 Citations

Scopus - 23 Citations

Scientific classification

FOS: Agrarian Sciences > Other Agrarian Sciences

Other information

Authenticus ID: P-002-RQB

DOI: 10.1039/c0ay00669f

Abstract (EN): The present work describes a simple and straightforward methodology for the determination of alpha-ketoglutaric acid (KA) and pyruvic acid (PA) in beer. This work involves three basic steps: (a) cleaning of the sample by solid phase extraction (SPE); (b) alpha-ketoacids' derivatization with o-phenylenediamine (OPDA) to form quinoxalines; and (c) separation and detection of the quinoxalines by high-performance liquid chromatography with spectrophotometric detection (HPLC-UV). The identity of the eluted compounds was also confirmed by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) in the positive ion mode. The developed methodology showed good repeatability (ca. 4%) and linearity as well as good limits of detection (0.091 and 0.055 mg L(-1) for KA and PA, respectively) and quantification (0.30 and 0.18 mg L(-1) for KA and PA, respectively). To sum up, the goal of this technical note is to develop a single HPLC procedure with previous SPE clean up suitable to use in routine analysis laboratories.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6