Application of gas-diffusion microextraction for high-performance liquid chromatographic analysis of aliphatic amines in fermented beverages

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Ines M Valente (Author) Other; Christiane M Santos (Author) Other; Luis M Goncalves (Author) FCUP; Jose A Rodrigues (Author) FCUP; Aquiles A Barros (Author) FCUP

Journal

Title: Analytical Methods

Vol. 4

Pages: 2569-2573

ISSN: 1759-9660

Publisher: Royal Society of Chemistry

Indexing

ISI Web of Knowledge - 20 Citations

Scopus - 21 Citations

Scientific classification

FOS: Agrarian Sciences > Other Agrarian Sciences

Other information

Authenticus ID: P-002-FCS

DOI: 10.1039/c2ay25272d

Abstract (EN): This work presents a novel application of a recently developed analytical extraction technique, gas-diffusion microextraction (GDME), in the analysis of three volatile aliphatic amines (methylamine, dimethylamine and ethylamine) in fermented beverages. The method involves the extraction of the amines using GDME and pre-column derivatisation of the collected amines with phenyl isothiocyanate (PITC) followed by analysis by high-performance liquid chromatography with UV detection. It was successfully applied to quantify the mentioned amines in Portuguese wines (white, red and rose) and beers (dark, pilsner and alcohol-free). Limits of detection ranged between 12 and 46 mu g L-1 and limits of quantification between 39 and 153 mu g L-1. This method is shown to be a competitive alternative to existing methods since it accomplishes a great simplification in the sample preparation needed.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5