Influence of batch or fed-batch growth on Staphylococcus epidermidis biofilm formation

• Type: Article in International Scientific Journal
• Date: 2004

Authors

Cerca, N (Author) Other; Pier, GB (Author) Other; Vilanova, M (Author) ICBAS; Oliveira, R (Author) Other; Azeredo, J (Author) Other

Journal

Title: Letters in Applied Microbiology

Vol. 39 No. 5

Pages: 420-424

ISSN: 0266-8254

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 31 Citations

Scopus - 32 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-000-D5B

DOI: 10.1111/j.1472-765x.2004.01601.x

Abstract (EN): Aims: To make a quantitative evaluation of the differences in biofilm formation by Staphylococcus epidermidis using batch and fed-batch growth systems and to correlate this with production of the major biofilm polysaccharide, poly-N-acetyl glucosamine (PNAG). Methods and Results: Dry weight measurements of biofilms formed in batch and fed-batch conditions were compared with haemagglutination titres, which measure the amount of PNAG produced. Strains grown in batch systems developed less biofilm than when grown in fed-batch systems. A good correlation was found between the amount of biofilm formed in fed-batch systems and the haemagglutination titres. Conclusions: Differences in biofilm formation and PNAG production by S. epidermidis are dependent on the availability of nutrients, with higher availability correlating with more biofilm and PNAG production. Significance of and Impact of the Study: Comparisons of the formation of biofilms by S. epidermidis are dependent on choosing an appropriate biofilm growth system. Comparability or disparity of conclusions among different investigations will be strongly influenced by which mode S. epidermidis biofilms are formed.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5