Synchrotron-based FTIR evaluation of biochemical changes in cancer and noncancer cells induced by brominated marine coelenteramine

• Type: Article in International Scientific Journal
• Date: 2023

Authors

Magalhaes, CM (Author) Other; Ducic, T (Author) Other; Pereira, RB (Author) Other; Gonzalez-Berdullas, P (Author) Other; Rodriguez Borges, JE (Author) FCUP; Pereira, DM (Author) Other; Joaquim C G E Esteves da Silva (Author) FCUP; Algarra, M (Author) Other; da Silva, LP (Author) FCUP

Journal

Title: Archives of Biochemistry and Biophysics

Vol. 743

ISSN: 0003-9861

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 0 Citations

Associated Projects

ChemiTumorTher - Quimioluminescência Marinha como uma Terapia Anticancro de Amplo Espectro com uma Selectividade e Actividade Tumoral Melhorada

Other information

Authenticus ID: P-00Y-GGJ

DOI: 10.1016/j.abb.2023.109660

Abstract (EN): The mode of action toward gastric cancer cells of brominated Coelenteramine, an analogue of a metabolic product of a marine bioluminescent reaction, was investigated by synchrotron radiation-based Fourier Transform Infrared spectrocopy (FTIR). This method revealed that the anticancer activity of brominated Coelenteramine is closely connected with cellular lipids, by affecting their organization and composition. More specifically, there is an increasing extent of oxidative stress, which results in changes in membrane polarity, lipid chain packing and lipid composition. However, this effect was not observed in a noncancer cell line, helping to explain its selectivity profile. Thus, synchrotron radiation-based FTIR helped to identify the potential of this Coelenteramine analogue in targeting membrane lipids, while proving to be a powerful technique to probe the mechanism of anticancer drugs.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6