Targetability of hyaluronic acid nanogel to cancer cells: In vitro and in vivo studies

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Pedrosa, SS (Author) Other; Pereira, P (Author) Other; Alexandra Correia (Author) FCUP; Gama, FM (Author) Other

Journal

Title: European Journal of Pharmaceutical Sciences

Vol. 104

Pages: 102-113

ISSN: 0928-0987

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 31 Citations

Scopus - 34 Citations

Other information

Authenticus ID: P-00M-NEK

DOI: 10.1016/j.ejps.2017.03.045

Abstract (EN): We have, in previous work developed, characterized and evaluated the biocompatibility of an engineered hyaluronic acid nanogel. Here we assess the targetability of a hyaluronic acid nanogel towards CD44 overexpressing cells, in vitro and in vivo. Results obtained by flow cytometry and confocal fluorescence microscopy shows that nanogel is greatly internalized by non-small cancer lung cells (A549 cells), that overexpress CD44 receptors. The biodistribution and tumor targetability of the nanogel labelled with a near infrared (NIR) probe were performed, in mice, through a non-invasive imaging system. Results revealed nanogel high targetability towards an induced subcutaneous A549 tumor. Nanogels pharmacoldnetics was evaluated also in healthy animals, and Alexa Fluor 680 labelled nanogel exhibited higher accumulation in liver, kidneys and skin. Also, a comparative biodistribution study was performed, using two NIR imaging probes, Cy5.5 and Alexa Fluor 680.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12