Green-Sustainable Recovery of Phenolic and Antioxidant Compounds from Industrial Chestnut Shells Using Ultrasound-Assisted Extraction: Optimization and Evaluation of Biological Activities In Vitro

• Type: Article in International Scientific Journal
• Date: 2020

Authors

Lameirao, F (Author) Other; Pinto, D (Author) Other; Vieira, EF (Author) Other; Peixoto, AF (Author) FCUP; Cristina Freire (Author) FCUP; Sut, S (Author) Other; Dall'Acqua, S (Author) Other; Paulo Costa (Author) FFUP; Delerue Matos, C (Author) Other; Rodrigues, F (Author) Other

Journal

Title: Antioxidants

Vol. 9

Final page: 267

ISSN: 2076-3921

Publisher: MDPI

Indexing

ISI Web of Knowledge - 31 Citations

Scopus - 34 Citations

Other information

Authenticus ID: P-00R-X57

DOI: 10.3390/antiox9030267

Abstract (EN): Chestnut processing industry generates large amounts of by-products, including leaves, burs and shells that are a source of bioactive compounds. The purpose of this study was to establish an ultrasound-assisted extraction (UAE) of phenolic and antioxidant compounds from industrial chestnut shells. A central composite design (CCD) was conducted to analyze the effects of time (4-46 min) and temperature (34-76 degrees C) in the antioxidant activity (2,2 '-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP)) and total phenolic compounds (TPC) of chestnut shells extracts. The optimal extraction conditions were obtained at 70 degrees C for 40 min. The optimal extract was characterized regarding phenolic profile, radical scavenging capacity, and effects on intestinal and dermal cell lines. The optimal extract revealed high amounts of ellagic acid (40.4 mu g/mg dw), followed by caffeic acid derivative (15.4 mu g/mg dw) and epigallocatechin (15.3 mu g/mg dw). Indeed, the extract exhibited the highest scavenging efficiencies against NOBLACK CIRCLE (IC50 = 0.1 mu g/mL) and HOCl (IC50 = 0.7 mu g/mL) and did not conducted to a decrease on HaCaT and HFF-1 viability up to 100 mu g/mL. Oppositely, a decrease on Caco-2 and HT29-MTX viability was observed. This study suggests that UAE could be a sustainable option to valorize chestnut shells as raw material for different industries.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 19